Hair Loss - Japanese study nizoral CREAM ie Ketoconazole
toppik hair fibers
Forum News Hair Transplant Hair Replacement Topical All Natural Drugs Hair Multiplication Gallery Female Hair Loss

Hair Loss

Forum Index   Personal Journal   Poll

Log in | User | Register

Search:  
Back to forum
Board view

 Dr. Klein Hair Regrowth Formula - clinically proven    

Japanese study nizoral CREAM ie Ketoconazole (Hair Loss Research & Clinical Trials)

posted by mjones, 04.11.2008, 15:20

» Your implication that the shampoo works better because of absorbing into
» a wet scalp, although on its face it may appear valid, does not hold true,
» since the study subjects all applied the cream immediately after
» shampooing, thus onto a WET SCALP,
»
»
» http://www.hairsite.com/hair-loss/forum_entry-id-6113-page-4-category-3-order-last_answer.html
»
» [image]
»
» Ketoconazole (KCZ), an imidazole anti-fungal agent, is known to be
» effective for the treatment of seborrheic dermatitis and dandruff. In
» addition, 2% KCZ shampoo was found to improve hair density and the size and
» proportion of anagen follicles in androgenetic alopecia (AGA) [1] and, in
» combination with finasteride, to have an additive effect for AGA [2].
» Recently, it has been reported that topical application of KCZ stimulates
» hair growth in C3H/HeN mice [3]. However, whether topical KCZ is effective
» enough to improve the clinical appearance of AGA is not yet clear. We
» therefore carried out an open trial of topical 2% KCZ lotion (Nizoral®) in
» combination with shampoos. Furthermore, to identify the mechanism, which
» can explain the clinical effect of KCZ on AGA, we performed transient
» transfection assays using CV-1 cells transfected with androgen receptors
» (AR).
»
» The six Japanese males from 23 to 51 years old were enrolled in this study
» with their written informed consent. They presented with grade II vertex to
» IVa AGA according to the Hamilton–Norwood classification [4]. The subjects
» applied topical 2% KCZ lotion (Nizoral®) almost every day during or
» immediately after hair washing with their own unmedicated shampoos. When
» they revisited our clinic every several months, clinical pictures were
» obtained to determine the efficacy of the treatment. Two of the men, one 23
» years old with grade II vertex and the other 25 years old with Va AGA,
» showed remarkable hair regrowth after 6 and 10 months, respectively (Fig.
» 1). The 23-year-old male stopped using KCZ and 3 months later hair loss
» recurrence on the vertex was noted (Fig. 1c). When he started using KCZ
» again during shampooing, hairs on the vertex grew again after 3 months
» (Fig. 1d). These findings constitute evidence of the clinical efficacy of
» KCZ for AGA. A 41-year-old male showed a slight increase in vertex hair
» growth after 1 year. Other three of the men, 31, 38 and 51 years old did
» not show significant improvement. These findings suggest that topical KCZ
» with shampoo can be effective for some males with AGA.
»
»
»
» Fig. 1. A 23-year-old Japanese man who used 2% ketoconazole (KCZ) lotion
» during shampooing everyday. Six months later, hair regrowth was attained
» (b) in comparison with the pre-treatment condition (a). Suspension of use
» for 3 months, however, caused recurrent hair loss (c). Renewed use of KCZ
» induced renewed growth of vertex hair (d). A 25-year-old Japanese man with
» AGA (e) applied 2% KCZ lotion immediately after shampooing everyday. Ten
» months later, hair regrowth was apparent (f).
»
» To identify the mechanism, which can explain the effect of KCZ on AGA, we
» performed transient transfection assays using an androgen-responsive
» synthetic promoter for CV-1 cells transfected with AR. At 50–70% confluency
» in a 24-well plate, the CV-1 cells, maintained in Dulbecco's modified
» Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, were
» transfected with the transfection reagent Fugene-6 (Roche Diagnostics
» Corp., Indianapolis, IN, USA) according to the manufacturer's instructions.
» For luciferase assays, we transfected 0.1μg of the reporter plasmid,
» pGL2-GRE3-bG-luc [5], 12.5ng of pCI-neo-BamX-AR(Gly23) [5] and 0.2μg
» of the pRL-CMV vector, the Renilla luciferase control reporter vector
» driven by the CMV immediate-early enhancer/promoter, as the internal
» controls. At 24h after transfection, we added fresh DMEM supplemented with
» 10% charcoal-treated fetal bovine serum with methyltrienolone (R1881, a
» stable synthetic androgen) or ethanol as a mock vehicle. The cells were
» also treated with KCZ (Janssen, L.P., Titusville, NJ) or ethanol as a mock
» vehicle. At 48h after transfection, the cells were harvested for luciferase
» assays. Luciferase activities were measured with a luminometer using the
» Dual-Luciferase™ reporter assay system (Promega, Madison, WI). The results
» were summarized from three independent sets of transfections and presented
» as mean±S.D.; statistical significance was tested with Student's t-test.
» The results demonstrated that 10 or 20μg/ml KCZ reduced luciferase
» activity to 67.5% (p<0.01) or 49.9% (p<0.03), respectively, reflecting its
» suppressive action on AR (Fig. 2). This finding suggests that KCZ improves
» AGA through the suppression of AR activity.
»
»
»
» Fig. 2. KCZ effect on R1881-induced AR transcactivity in CV-1 cells
» transfected with AR. We used the transfection reagent Fugene-6 (Roche
» Diagnostics Corp., Indianapolis, IN, USA) to transfect 0.1&#956;g of the
» reporter plasmid, pGL2-GRE3-bG-luc, 12.5ng of pCI-neo-BamX-AR(Gly23) and
» 0.2&#956;g of pRL-CMV vector (lanes 1–6) into CV-1 cells cultured at 50–70%
» confluency in a 24-well plate. The infected cells were treated with
» 10&#8722;9M R1881 (lanes 2–6) or ethanol as a mock vehicle (lane 1). After
» overnight incubation, KCZ at the indicated concentration was added to the
» cultures. The activities of the various reporter genes were compared with
» the luciferase activity in the presence of R1881 and the absence of KCZ
» (lane 2).
»
» Dermal papilla cells are the main targets for androgen in hair follicles,
» as evidenced by immunohistochemistry [6] and reporter assays [7] for the
» detection of AR. Deep penetration of KCZ into dermal papilla is therefore
» necessary to realize the suppressive action on androgen in vivo. The use of
» KCZ in combination with detergent containing shampoos in this study may
» enhance KCZ penetration. On the other hand, a recent study demonstrated
» that KCZ stimulates hair growth in mice [3], suggesting that its effect on
» hair is androgen independent. To summarize, KCZ may exert its effect on AGA
» in both an androgen-dependent and -independent manner.
»
»
»
»
» References
» return to Article Outline
»
» [1]. [1]Pierard-Franchimont C, De Doncker P, Cauwenbergh G, Pierard GE.
» Ketoconazole shampoo: effect of long-term use in androgenic alopecia.
» Dermatology. 1998;196:474–477. MEDLINE | CrossRef
»
» [2]. [2]Khandpur S, Suman M, Reddy BS. Comparative efficacy of various
» treatment regimens for androgenetic alopecia in men. J Dermatol.
» 2002;29:489–498. MEDLINE
»
» [3]. [3]Jiang J, Tsuboi R, Kojima Y, Ogawa H. Topical application of
» ketoconazole stimulates hair growth in C3H/HeN mice. J Dermatol.
» 2005;32:243–247. MEDLINE
»
» [4]. [4]Norwood OT. Male pattern baldness: classification and incidence.
» South Med J. 1975;68:1359–1365. MEDLINE
»
» [5]. [5]Inui S, Nakao T, Itami S. Modulation of androgen receptor
» transcriptional activity by anti-acne reagents. J Dermatol Sci.
» 2004;36:97–101. Abstract | Full Text | Full-Text PDF (135 KB) | MEDLINE |
» CrossRef
»
» [6]. [6]Itami S, Kurata S, Sonoda T, Takayasu S. Interaction between
» dermal papilla cells and follicular epithelial cells in vitro: effect of
» androgen. Br J Dermatol. 1995;132:527–532. MEDLINE
»
» [7]. [7]Inui S, Itami S, Pan HJ, Chang C. Lack of androgen transcriptional
» activity in human keratinocytes. J Dermatol Sci. 2000;23:87–92. Abstract |
» Full Text | Full-Text PDF (106 KB) | MEDLINE | CrossRef

So was this the shampoo they used that grew the hair or the cream after?? I use 2% Keto for like 2 weeks now..3x a week...makes my hair thicker and fuller....but do i need to use a cream nizoral after?? Can some one explain it to me....??


mjones is located in [NA] and he is available to meet: NO

Post reply
 

Complete thread:

Back to forum
Board view
56966 Postings in 5271 Threads, 2182 registered users
Hair Loss | Admin contact
| FORUM POLICY