Hair Loss - Japanese study nizoral CREAM ie Ketoconazole
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Japanese study nizoral CREAM ie Ketoconazole (Hair Loss Research & Clinical Trials)

posted by mjones, 04.11.2008, 15:20

» Your implication that the shampoo works better because of absorbing into
» a wet scalp, although on its face it may appear valid, does not hold true,
» since the study subjects all applied the cream immediately after
» shampooing, thus onto a WET SCALP,
»
»
» http://www.hairsite.com/hair-loss/forum_entry-id-6113-page-4-category-3-order-last_answer.html
»
» [image]
»
» Ketoconazole (KCZ), an imidazole anti-fungal agent, is known to be
» effective for the treatment of seborrheic dermatitis and dandruff. In
» addition, 2% KCZ shampoo was found to improve hair density and the size and
» proportion of anagen follicles in androgenetic alopecia (AGA) [1] and, in
» combination with finasteride, to have an additive effect for AGA [2].
» Recently, it has been reported that topical application of KCZ stimulates
» hair growth in C3H/HeN mice [3]. However, whether topical KCZ is effective
» enough to improve the clinical appearance of AGA is not yet clear. We
» therefore carried out an open trial of topical 2% KCZ lotion (Nizoral®) in
» combination with shampoos. Furthermore, to identify the mechanism, which
» can explain the clinical effect of KCZ on AGA, we performed transient
» transfection assays using CV-1 cells transfected with androgen receptors
» (AR).
»
» The six Japanese males from 23 to 51 years old were enrolled in this study
» with their written informed consent. They presented with grade II vertex to
» IVa AGA according to the Hamilton–Norwood classification [4]. The subjects
» applied topical 2% KCZ lotion (Nizoral®) almost every day during or
» immediately after hair washing with their own unmedicated shampoos. When
» they revisited our clinic every several months, clinical pictures were
» obtained to determine the efficacy of the treatment. Two of the men, one 23
» years old with grade II vertex and the other 25 years old with Va AGA,
» showed remarkable hair regrowth after 6 and 10 months, respectively (Fig.
» 1). The 23-year-old male stopped using KCZ and 3 months later hair loss
» recurrence on the vertex was noted (Fig. 1c). When he started using KCZ
» again during shampooing, hairs on the vertex grew again after 3 months
» (Fig. 1d). These findings constitute evidence of the clinical efficacy of
» KCZ for AGA. A 41-year-old male showed a slight increase in vertex hair
» growth after 1 year. Other three of the men, 31, 38 and 51 years old did
» not show significant improvement. These findings suggest that topical KCZ
» with shampoo can be effective for some males with AGA.
»
»
»
» Fig. 1. A 23-year-old Japanese man who used 2% ketoconazole (KCZ) lotion
» during shampooing everyday. Six months later, hair regrowth was attained
» (b) in comparison with the pre-treatment condition (a). Suspension of use
» for 3 months, however, caused recurrent hair loss (c). Renewed use of KCZ
» induced renewed growth of vertex hair (d). A 25-year-old Japanese man with
» AGA (e) applied 2% KCZ lotion immediately after shampooing everyday. Ten
» months later, hair regrowth was apparent (f).
»
» To identify the mechanism, which can explain the effect of KCZ on AGA, we
» performed transient transfection assays using an androgen-responsive
» synthetic promoter for CV-1 cells transfected with AR. At 50–70% confluency
» in a 24-well plate, the CV-1 cells, maintained in Dulbecco's modified
» Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, were
» transfected with the transfection reagent Fugene-6 (Roche Diagnostics
» Corp., Indianapolis, IN, USA) according to the manufacturer's instructions.
» For luciferase assays, we transfected 0.1μg of the reporter plasmid,
» pGL2-GRE3-bG-luc [5], 12.5ng of pCI-neo-BamX-AR(Gly23) [5] and 0.2μg
» of the pRL-CMV vector, the Renilla luciferase control reporter vector
» driven by the CMV immediate-early enhancer/promoter, as the internal
» controls. At 24h after transfection, we added fresh DMEM supplemented with
» 10% charcoal-treated fetal bovine serum with methyltrienolone (R1881, a
» stable synthetic androgen) or ethanol as a mock vehicle. The cells were
» also treated with KCZ (Janssen, L.P., Titusville, NJ) or ethanol as a mock
» vehicle. At 48h after transfection, the cells were harvested for luciferase
» assays. Luciferase activities were measured with a luminometer using the
» Dual-Luciferase™ reporter assay system (Promega, Madison, WI). The results
» were summarized from three independent sets of transfections and presented
» as mean±S.D.; statistical significance was tested with Student's t-test.
» The results demonstrated that 10 or 20μg/ml KCZ reduced luciferase
» activity to 67.5% (p<0.01) or 49.9% (p<0.03), respectively, reflecting its
» suppressive action on AR (Fig. 2). This finding suggests that KCZ improves
» AGA through the suppression of AR activity.
»
»
»
» Fig. 2. KCZ effect on R1881-induced AR transcactivity in CV-1 cells
» transfected with AR. We used the transfection reagent Fugene-6 (Roche
» Diagnostics Corp., Indianapolis, IN, USA) to transfect 0.1&#956;g of the
» reporter plasmid, pGL2-GRE3-bG-luc, 12.5ng of pCI-neo-BamX-AR(Gly23) and
» 0.2&#956;g of pRL-CMV vector (lanes 1–6) into CV-1 cells cultured at 50–70%
» confluency in a 24-well plate. The infected cells were treated with
» 10&#8722;9M R1881 (lanes 2–6) or ethanol as a mock vehicle (lane 1). After
» overnight incubation, KCZ at the indicated concentration was added to the
» cultures. The activities of the various reporter genes were compared with
» the luciferase activity in the presence of R1881 and the absence of KCZ
» (lane 2).
»
» Dermal papilla cells are the main targets for androgen in hair follicles,
» as evidenced by immunohistochemistry [6] and reporter assays [7] for the
» detection of AR. Deep penetration of KCZ into dermal papilla is therefore
» necessary to realize the suppressive action on androgen in vivo. The use of
» KCZ in combination with detergent containing shampoos in this study may
» enhance KCZ penetration. On the other hand, a recent study demonstrated
» that KCZ stimulates hair growth in mice [3], suggesting that its effect on
» hair is androgen independent. To summarize, KCZ may exert its effect on AGA
» in both an androgen-dependent and -independent manner.
»
»
»
»
» References
» return to Article Outline
»
» [1]. [1]Pierard-Franchimont C, De Doncker P, Cauwenbergh G, Pierard GE.
» Ketoconazole shampoo: effect of long-term use in androgenic alopecia.
» Dermatology. 1998;196:474–477. MEDLINE | CrossRef
»
» [2]. [2]Khandpur S, Suman M, Reddy BS. Comparative efficacy of various
» treatment regimens for androgenetic alopecia in men. J Dermatol.
» 2002;29:489–498. MEDLINE
»
» [3]. [3]Jiang J, Tsuboi R, Kojima Y, Ogawa H. Topical application of
» ketoconazole stimulates hair growth in C3H/HeN mice. J Dermatol.
» 2005;32:243–247. MEDLINE
»
» [4]. [4]Norwood OT. Male pattern baldness: classification and incidence.
» South Med J. 1975;68:1359–1365. MEDLINE
»
» [5]. [5]Inui S, Nakao T, Itami S. Modulation of androgen receptor
» transcriptional activity by anti-acne reagents. J Dermatol Sci.
» 2004;36:97–101. Abstract | Full Text | Full-Text PDF (135 KB) | MEDLINE |
» CrossRef
»
» [6]. [6]Itami S, Kurata S, Sonoda T, Takayasu S. Interaction between
» dermal papilla cells and follicular epithelial cells in vitro: effect of
» androgen. Br J Dermatol. 1995;132:527–532. MEDLINE
»
» [7]. [7]Inui S, Itami S, Pan HJ, Chang C. Lack of androgen transcriptional
» activity in human keratinocytes. J Dermatol Sci. 2000;23:87–92. Abstract |
» Full Text | Full-Text PDF (106 KB) | MEDLINE | CrossRef

So was this the shampoo they used that grew the hair or the cream after?? I use 2% Keto for like 2 weeks now..3x a week...makes my hair thicker and fuller....but do i need to use a cream nizoral after?? Can some one explain it to me....??


mjones is located in [NA] and he is available to meet: NO

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